KevinCompounds said:My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed: Main peak retention time:…
Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in the refrigerator.
Day 1: 99.6% purity
Day 14: 98.6% purity
Day 28: 98.4% purity
Minimal degradation over 28 days when properly refrigerated. This confirms the standard 28-day beyond-use date is appropriate. Beyond 28 days, I'd reconstitute a fresh vial to be safe.
One thing that is still open after KevinCompounds’s answer:
How long did you give it before you decided it was working?
hank_denver said:Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in…
I tested my certificates of analysis batch through Janoshik — 98.7% purity. That's within acceptable range for compounded peptides. Turnaround was 8 business days.
The test costs ~$139 but gives you actual HPLC chromatography data plus mass spec confirmation. For the peace of mind alone, it's worth every penny. I test every new batch from a new vendor.
Sigma-Aldrich — Research-Grade Standards
Certified reference materials, analytical reagents, and research-grade standards for peptide verification. Trusted by laboratories worldwide.
Shop Reference Standardshank_denver said:Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in…
That is right, and it stops being right at the edges. The general case is well behaved; the interesting cases in this thread are all at the boundary where the general case breaks.
Ask again with the specifics and you will get a better answer than this one.
hank_denver said:Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in…
Thank you — that is the clearest version of this I have read, and I have read a lot of them. Taking it to my next appointment.