paige_pharma said:COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis: No HPLC data: If there's no…
HPLC results interpretation for certificates of analysis: when you get your Janoshik report back, here's what the numbers mean:
- Purity %: Should be >97%. Below 95% is concerning.
- Retention time: Should match reference standard for the peptide
- Related substances: Degradation products should be <2% total
- Single impurity: No individual impurity should exceed 1%
My last batch: 99.6% purity, 0.0% total related substances. Clean chromatogram with single sharp peak at expected retention time. Exactly what you want to see.
KetoKyle said:One addition: an unstated method makes a report close to uninterpretable.
Thank you — that is the clearest version of this I have read, and I have read a lot of them. Printing the relevant bit and taking it with me.
paige_pharma said:COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis: No HPLC data: If there's no…
I tested my certificates of analysis batch through Janoshik — 98.1% purity. That's within acceptable range for compounded peptides. Turnaround was 14 business days.
The test costs ~$136 but gives you actual HPLC chromatography data plus mass spec confirmation. For the peace of mind alone, it's worth every penny. I test every new batch from a new vendor.
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Shop Reference Standardssteph_laguna said:Two reports on the same product from two laboratories, four percentage points apart, and no method stated on either.
Reconstituted peptide stability data for certificates of analysis: I had Janoshik test my reconstituted vial at day 1, day 14, and day 28 stored in the refrigerator.
Day 1: 99.4% purity
Day 14: 98.4% purity
Day 28: 99.2% purity
Minimal degradation over 28 days when properly refrigerated. This confirms the standard 28-day beyond-use date is appropriate. Beyond 28 days, I'd reconstitute a fresh vial to be safe.
Moderator note: reminder that nothing in this thread is medical advice, and that clinical claims need a source. No action needed from anybody.