TrialNerd_Beth said:Do it in two steps and it stops being confusing.
I am going to disagree with reconstituting low as a general rule. More diluent, more punctures, more in-use days at room temperature, and the stability trade-off is real. Precision is not the only variable being optimised.
The figures, for anyone assembling their own picture. Practical: unopened, refrigerated at 2 to 8°C; in use, refrigerated and used within the preservative-limited window; never frozen; and a visual check every time — a haze that does not settle is a reason to stop, not to wonder.
DataDave said:I am going to disagree with reconstituting low as a general rule.
There is a second half to this that has not been said yet. Freezing is the one to avoid, and freeze-thaw more so. Ice-crystal formation and the concentration changes at the phase boundary drive aggregation, and aggregated peptide does not recover on thawing. A vial that has been frozen and thawed is not rescued by returning it to the fridge.
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View ResultsA narrower follow-up, since the general answer is now clear:
How much material I am losing to dead space, and whether that explains why a 10mg vial gives me nine usable draws rather than ten?