Dr.GastroMayo said:Inter-laboratory variation comes from real methodological differences, not sloppiness: which reference standard was used and how it was itself…
Vendor quality matters when discussing certificates of analysis. I've tested my last 5 batches through Janoshik and purity has ranged from 99.0% to 99.6%. Consistency is key.
For anyone new: always request the COA, and consider independent verification. The $300 for a Janoshik test is worth the peace of mind.
A narrower follow-up, since the general answer is now clear:
Why two laboratories report different purity for the same batch, and which number to believe?
hank_denver said:Vendor quality matters when discussing certificates of analysis.
My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed:
Main peak retention time: 14.4 minutes (consistent with semaglutide reference standard)
Peak purity: 99.6%
Related substances: 0.6% (single impurity at 8.7 minutes — likely a synthesis intermediate)
Mass spec: m/z 4113.58 (matches expected molecular weight)
This is a clean result. The mass spec confirmation is particularly important — it proves the molecule is actually semaglutide and not a substitution.
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View ResultsClosing the loop on my own question.
Purity and content being different measurements was the piece I was missing. The vial was overfilled and pure, which is a very different problem from the one I thought I had.
NurseKim_ATL said:My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed: Main peak retention time:…
Agreed, and mass spec answers a question purity does not: identity. High purity tells you one thing dominates the chromatogram, not that it is the molecule you ordered. A mass within about a dalton of expected is the identity check.